OrangerV1, Main, Exploration, bibRecord, 000D42

Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.

Identifieur interne : 000D42 ( Main/Exploration ); précédent : 000D41; suivant : 000D43

Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.

Auteurs : Marina M. Vdovenko [Russie] ; Alexandra S. Stepanova ; Sergei A. Eremin ; Nguyen Van Cuong ; Natalia A. Uskova ; Ivan Yu Sakharov

Source :

Food chemistry [ 0308-8146 ] ; 2013.

RBID : pubmed:23790860

English descriptors

KwdEn :
- 2,4-Dichlorophenoxyacetic Acid (analysis), Citrus sinensis (chemistry), Drug Residues (analysis), Enzyme-Linked Immunosorbent Assay (methods), Fruit (chemistry), Herbicides (analysis), Limit of Detection, Luminescent Measurements (methods).
MESH :
- chemical , analysis : 2,4-Dichlorophenoxyacetic Acid, Herbicides.
- analysis : Drug Residues.
- chemistry : Citrus sinensis, Fruit.
- methods : Enzyme-Linked Immunosorbent Assay, Luminescent Measurements.
- Limit of Detection.

Abstract

Direct competitive enzyme-linked immunosorbent assay (ELISA) for 2,4-dichlorophenoxyacetic acid (2,4-D) was developed. Varying the concentrations of monoclonal anti-2,4-D-antibody and the conjugate of soybean peroxidase and 2,4-D the conditions of ELISA performance were optimised. The chemiluminescent method based on peroxidase-catalysed oxidation of luminol was applied to measure the enzyme activity of the conjugate. A mixture of 3-(10'-phenothiazinyl)propane-1-sulfonate and 4-morpholinopyridine was used as potent enhancer of chemiluminescence signal. It was shown that the values of the lower detection limit, IC50 and the working range were 1.5, 64.0, and 6.5-545ng/mL, respectively. The recovery values of CL-ELISA from 10 spiked samples of oranges (n=5) and mandarins (n=5) cultivated in green house without use of 2,4-D and containing different 2,4-D concentrations (10-300ng/mL) were ranged from 92% to 104% that indicated on the absence of matrix effect for the fruit extracts of interest. Determination of 2,4-D in peel of five oranges and five mandarins purchased from stores in Vietnam showed that 2,4-D content in oranges fruits (79-104μg/kg) was significantly higher than that in mandarins (1.66-2.82μg/kg).

DOI: 10.1016/j.foodchem.2013.04.060
PubMed: 23790860

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">Direct competitive enzyme-linked immunosorbent assay (ELISA) for 2,4-dichlorophenoxyacetic acid (2,4-D) was developed. Varying the concentrations of monoclonal anti-2,4-D-antibody and the conjugate of soybean peroxidase and 2,4-D the conditions of ELISA performance were optimised. The chemiluminescent method based on peroxidase-catalysed oxidation of luminol was applied to measure the enzyme activity of the conjugate. A mixture of 3-(10'-phenothiazinyl)propane-1-sulfonate and 4-morpholinopyridine was used as potent enhancer of chemiluminescence signal. It was shown that the values of the lower detection limit, IC50 and the working range were 1.5, 64.0, and 6.5-545ng/mL, respectively. The recovery values of CL-ELISA from 10 spiked samples of oranges (n=5) and mandarins (n=5) cultivated in green house without use of 2,4-D and containing different 2,4-D concentrations (10-300ng/mL) were ranged from 92% to 104% that indicated on the absence of matrix effect for the fruit extracts of interest. Determination of 2,4-D in peel of five oranges and five mandarins purchased from stores in Vietnam showed that 2,4-D content in oranges fruits (79-104μg/kg) was significantly higher than that in mandarins (1.66-2.82μg/kg).</div>
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Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.

Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.

Source :

English descriptors

Abstract

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